antibodies against slc8a1  (Proteintech)

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis demonstrated that genetic susceptibility to SLC8A1 eQTL may accelerate TD progression

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies against SLC8A1 and GAPDH, both at a 1:2000 dilution (Proteintech, Wuhan, China; catalog numbers 12359-1-AP and 60004-1-Ig, respectively).

Techniques:

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: Transcriptome sequencing data analysis confirmed that genetic susceptibility to SLC8A1 eQTL contributed to TD progression

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies against SLC8A1 and GAPDH, both at a 1:2000 dilution (Proteintech, Wuhan, China; catalog numbers 12359-1-AP and 60004-1-Ig, respectively).

Techniques: Sequencing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: The experiments verified that SLC8A1 promoted progression of TD. ( A ) Photos of the Achilles tendon in control and tendinopathy groups. ( B ) H&E and Masson’s staining for control and TD tendon. Scale bar: 200 μm. ( C ) Immunofluorescence staining for SLC8A1 in control and TD tissues. Scale bar: 100 μm. ( D ) Western blotting was used to detect SLC8A1 protein levels in tendon tissues of the normal group and TD group. ( E ) qRT-PCR analysis of the mRNA expression of SLC8A1 in TD groups. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies against SLC8A1 and GAPDH, both at a 1:2000 dilution (Proteintech, Wuhan, China; catalog numbers 12359-1-AP and 60004-1-Ig, respectively).

Techniques: Control, Staining, Immunofluorescence, Western Blot, Quantitative RT-PCR, Expressing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis showed that genetic susceptibility to SLC8A1 eQTL was associated with reduced levels of gamma − glutamylisoleucine

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies against SLC8A1 and GAPDH, both at a 1:2000 dilution (Proteintech, Wuhan, China; catalog numbers 12359-1-AP and 60004-1-Ig, respectively).

Techniques:

Journal: BioMed Research International

Article Title: Silencing lncRNA 93358 Inhibits the Apoptosis of Myocardial Cells in Myocardial Infarction Rats by Inducing the Expression of SLC8A1

doi: 10.1155/2022/1138709

Figure Lengend Snippet: Validation of sequencing data. (a and b) The expression levels of lncRNA 93358, SLC8A1, and TRPS1 (a) and miR-34a-3p, miR-125b-2-3p, and miR-466c-3p (b) in the rat myocardial tissues were detected by RT-qPCR. ∗∗ P < 0.01.

Article Snippet: Then, the membrane was incubated with the primary antibody against SLC8A1 (1 : 1000, 55075-1-AP, Proteintech, Chicago, USA), Bcl-2 (1 : 1000, bs-34012R, Bioss, Beijing, China), Bax (1 : 1000, 50599-2-lg, Proteintech, Chicago, USA), and Tubulin (1 : 1000, 10094-1-AP, Proteintech, Chicago, USA).

Techniques: Biomarker Discovery, Sequencing, Expressing, Quantitative RT-PCR

Journal: BioMed Research International

Article Title: Silencing lncRNA 93358 Inhibits the Apoptosis of Myocardial Cells in Myocardial Infarction Rats by Inducing the Expression of SLC8A1

doi: 10.1155/2022/1138709

Figure Lengend Snippet: The regulation axis of lncRNA 93358/miRNA/SLC8A1. (a and b) The expression of lncRNA 93358 and miR-466c-3p in the four rat myocardial tissues was measured by RT-qPCR. ∗ P < 0.05 vs. control, # P < 0.05 vs. model, and ^ P < 0.05 vs. NC group. (c) The binding between SLC8A1 and miR-466c-3p was measured by dual-luciferase reporter. ∗ P < 0.05.

Article Snippet: Then, the membrane was incubated with the primary antibody against SLC8A1 (1 : 1000, 55075-1-AP, Proteintech, Chicago, USA), Bcl-2 (1 : 1000, bs-34012R, Bioss, Beijing, China), Bax (1 : 1000, 50599-2-lg, Proteintech, Chicago, USA), and Tubulin (1 : 1000, 10094-1-AP, Proteintech, Chicago, USA).

Techniques: Expressing, Quantitative RT-PCR, Control, Binding Assay, Luciferase

Journal: BioMed Research International

Article Title: Silencing lncRNA 93358 Inhibits the Apoptosis of Myocardial Cells in Myocardial Infarction Rats by Inducing the Expression of SLC8A1

doi: 10.1155/2022/1138709

Figure Lengend Snippet: Effect of lncRNA 93358 on apoptosis in myocardial infarction rat cardiomyocytes. (a) The apoptosis of myocardial tissues was determined by TUNEL assay. (b and c) The expression level of Bax, Bcl-2, and SLC8A1 in the myocardial cells was determined by RT-qPCR (b) and Western blotting (c). ∗ P < 0.05 vs. control, # P < 0.05 vs. model, and ^ P < 0.05 vs. NC group.

Article Snippet: Then, the membrane was incubated with the primary antibody against SLC8A1 (1 : 1000, 55075-1-AP, Proteintech, Chicago, USA), Bcl-2 (1 : 1000, bs-34012R, Bioss, Beijing, China), Bax (1 : 1000, 50599-2-lg, Proteintech, Chicago, USA), and Tubulin (1 : 1000, 10094-1-AP, Proteintech, Chicago, USA).

Techniques: TUNEL Assay, Expressing, Quantitative RT-PCR, Western Blot, Control